[原分所] IAMS Lecture Announcement on February 20, 3:30PM (Thursday)----Prof. ERik Snapp
消息來源：原分所
截止日期：2014-02-14
IAMS Lecture Announcement
中研院原分所演講公告
Title: Visualizing Misfolded Protein Stress in Living Cells and Why You Should Stop Using EGFP
Speaker: Prof. ERik Snapp (Albert Einstein College of Medicine, USA)
Time: 3:30PM, February 20 (Thursday)
Place: Dr. Poe Lecture Hall, IAMS (本所浦大邦講堂 臺大校園內)
Contact: Dr. Kaito Takahashi 高橋開人博士
Abstract:
Fluorescent proteins (FPs) have been powerful tools for cell biologists for over 15 years. The large variety of FPs
available rarely comes with an instruction manual or a warning label. The potential pitfalls of the use of FPs in
cellular organelles represent a significant concern for investigators. FPs generally did not evolve in the often
distinctive physicochemical environments of subcellular organelles. In organelles, FPs can misfold, go dark, and
even distort organelle morphology. In this seminar, I will describe the issues associated with FPs in organelles
and provide solutions to enable investigators to better exploit FP technology in cells. I will then describe how we have
used FP fusions and biophysical fluorescence microscopy techniques (photobleaching, FRAP, and FLIP) to study
the organization and function of chaperones in the endoplasmic reticulum during stress.
中研院原分所演講公告
Title: Visualizing Misfolded Protein Stress in Living Cells and Why You Should Stop Using EGFP
Speaker: Prof. ERik Snapp (Albert Einstein College of Medicine, USA)
Time: 3:30PM, February 20 (Thursday)
Place: Dr. Poe Lecture Hall, IAMS (本所浦大邦講堂 臺大校園內)
Contact: Dr. Kaito Takahashi 高橋開人博士
Abstract:
Fluorescent proteins (FPs) have been powerful tools for cell biologists for over 15 years. The large variety of FPs
available rarely comes with an instruction manual or a warning label. The potential pitfalls of the use of FPs in
cellular organelles represent a significant concern for investigators. FPs generally did not evolve in the often
distinctive physicochemical environments of subcellular organelles. In organelles, FPs can misfold, go dark, and
even distort organelle morphology. In this seminar, I will describe the issues associated with FPs in organelles
and provide solutions to enable investigators to better exploit FP technology in cells. I will then describe how we have
used FP fusions and biophysical fluorescence microscopy techniques (photobleaching, FRAP, and FLIP) to study
the organization and function of chaperones in the endoplasmic reticulum during stress.