[原分所演講] IAMS Lecture on August 30, 10:00am, Dr. Poe Lecture Hall, Dr. Wan-Chen Lin
消息來源：化學系辦公室
截止日期：2016-08-24
IAMS Lecture Announcement
中研院原分所演講公告
Title: Optogenetic manipulation of neural inhibition: from tool development to in vivo photo-control
Speaker: Dr. Wan-Chen Lin (Department of Molecular and Cell Biology, University of California, Berkeley, USA)
Time: 10:00 AM, August 30 (Tuesday)
Place: Dr. Poe Lecture Hall, IAMS (本所浦大邦講堂 臺大校園內)
Contact: Dr. Jung-Chi Liao 廖仲麒博士
Abstract: Optogenetics is a hybrid approach that integrates optical and genetic manipulations of a protein target to control the physiology of a cell. It is a revolutionary technique for functional investigations of signaling and circuitry in the nervous system. The use of light allows remote control of protein function with high spatial and temporal precision. I will present a chemical-genetic method for photo-sensitizing GABAA receptors, the major mediators of inhibitory neurotransmission in the brain. Each light-regulated GABAA receptor (LiGABAR) was engineered by conjugating an azobenzene-based photoswitch ligand nearby the agonist-binding site. In concert with the photo-isomerization of azobenzene, a LiGABAR can be rapidly and reversibly switched between normal and antagonized states by two different wavelengths of light. This approach has been applied to confer light sensitivity onto six subtypes of GABAA receptors individually, providing photo-control with refined biochemical specificity. LiGABARs have been successfully implemented ex vivo in brain slices and in vivo in the cerebral cortex of awake mice, enabling optical manipulation of inhibitory synaptic transmission, neuronal firing, and vision-evoked cortical activities. The LiGABAR toolkit thus offers a powerful means for optogenetic interrogations of GABAergic signaling in the complex nervous system.
中研院原分所演講公告
Title: Optogenetic manipulation of neural inhibition: from tool development to in vivo photo-control
Speaker: Dr. Wan-Chen Lin (Department of Molecular and Cell Biology, University of California, Berkeley, USA)
Time: 10:00 AM, August 30 (Tuesday)
Place: Dr. Poe Lecture Hall, IAMS (本所浦大邦講堂 臺大校園內)
Contact: Dr. Jung-Chi Liao 廖仲麒博士
Abstract: Optogenetics is a hybrid approach that integrates optical and genetic manipulations of a protein target to control the physiology of a cell. It is a revolutionary technique for functional investigations of signaling and circuitry in the nervous system. The use of light allows remote control of protein function with high spatial and temporal precision. I will present a chemical-genetic method for photo-sensitizing GABAA receptors, the major mediators of inhibitory neurotransmission in the brain. Each light-regulated GABAA receptor (LiGABAR) was engineered by conjugating an azobenzene-based photoswitch ligand nearby the agonist-binding site. In concert with the photo-isomerization of azobenzene, a LiGABAR can be rapidly and reversibly switched between normal and antagonized states by two different wavelengths of light. This approach has been applied to confer light sensitivity onto six subtypes of GABAA receptors individually, providing photo-control with refined biochemical specificity. LiGABARs have been successfully implemented ex vivo in brain slices and in vivo in the cerebral cortex of awake mice, enabling optical manipulation of inhibitory synaptic transmission, neuronal firing, and vision-evoked cortical activities. The LiGABAR toolkit thus offers a powerful means for optogenetic interrogations of GABAergic signaling in the complex nervous system.