IAMS Lecture Announcement 中研院原分所演講公告

Title: The Story of Photonics and Single Molecules, from Early FM Spectroscopy in Solids, to Super-Resolution Nanoscopy in Cells and Beyond

Speaker: Prof. William E. Moerner (Departments of Chemistry and Applied Physics, Stanford University, USA)

Time: 13:30, January 28 (Monday), 2019

Place: Dr. Poe Lecture Hall, IAMS (本所浦大邦紀念講堂，臺大校園內)

Contact: Dr. Chia-Lung Hsieh 謝佳龍博士

Abstract:

Roughly 30 years ago, low temperature experiments aimed at establishing the ultimate limits to optical storage in solids led to the first optical detection and spectroscopy of a single molecule in the condensed phase.  At this unexplored ultimate limit, many surprises occurred where single molecules showed both spontaneous changes (blinking) and light-driven control of emission, properties that were also observed in 1997 at room temperature with single green fluorescent protein variants. In 2006, PALM and subsequent approaches showed that the optical diffraction limit of ~200 nm can be circumvented to achieve super-resolution fluorescence microscopy, or nanoscopy, with relatively nonperturbative visible light.  Essential to this is the combination of single-molecule fluorescence imaging with active control of the emitting concentration and sequential localization of single fluorophores decorating a structure.  Super-resolution microscopy has opened up a new frontier in which biological structures and behavior can be observed in live cells with resolutions down to 20-40 nm and below.  Current methods development research addresses ways to extract more information from each single molecule such as 3D position and orientation, and to assure not only precision, but also accuracy. In cells, a tilted light sheet design enables 3D super-resolution in thick samples, and low temperature imaging shows promise for improved ground truth localizations.  Still, it is worth noting that in spite of all the interest in super-resolution, even in the “conventional” single-molecule tracking regime where the motions of individual biomolecules are recorded in solution or in cells rather than the shapes of extended structures, much can be learned about biological processes when ensemble averaging is removed. Moreover, when single molecules are trapped in solution for extended study, hidden dynamics can be uncovered such as for individual photosynthetic proteins interacting with quenchers.

報名網址: https://ppt.cc/fqEXkx       聯絡人:劉嘉琪助理 ccliu@pub.iams.sinica.edu.tw