IAMS Lecture Announcement
中研院原分所演講公告

Title: Plasmonic nanoprobes visualize dynamic motions of protein molecular motors
Speaker: Dr. Ryota Iino (Institute for Molecular Science, National Institutes of Natural Sciences, Japan)

Time: 2:30 PM, August 7 (Wednesday), 2019
Place: C.T. Chang Memorial Hall (4F張昭鼎紀念講堂), IAMS

Contact: Dr. Chia-Lung Hsieh

Abstract:

To understand operation and design principles of protein molecular motors [1], we have been developing high-precison and high-speed single-molecule imaging methods visualizing fast and free motions of protein molecular motors with angstrom localization precision and microsecond temporal resolution [2]. Our methods are based on dark-field scattering imaging of plasmonic nanoprobes such as gold nanoparticles [3] and nanorods [4]. As probes of single-molecule imaging, they have advantages over commonly used ones such as fluorescent dyes and quantum dots, because much stronger signals can be obtained without suffering from photobleaching and blinking. In my talk, I will discuss chemo-mechanical mechanisms and coordination mechanisms of linear molecular motors kinesin-1 [5], dynein, processive chitinase [6], and a rotary molecular motor V₁-ATPase [7-9], revealed by our single-molecule imaging methods. I will also introduce our recent approaches to realize multi-color single-molecule imaging with gold, silver, and gold-silver alloy nanoparticles [10], and to decrease the probe size down to 10 nm.

Keywords: Protein molecular motors, Chemo-mechanical energy conversion, Single-molecule imaging, Plasmonic nanoparticles, Dark-field imaging

References

1. Iino et al., Biochim Biophys Acta 1862, 241-252 (2018)
2. Ando et al., Biophys. J. 115, 2413-2427 (2018)
3. Ueno et al., Biophys. J. 98, 2014-2023 (2010)
4. Enoki et al., Anal. Chem. 87, 2079-2086 (2015)
5. Isojima and R. Iino et al., Nat. Chem. Biol. 12, 290-297 (2016)
6. Nakamura et al., Nat. Commun. 9, 3814 (2018)
7. Minagawa and H. Ueno et al., J. Biol. Chem. 288, 32700-32707 (2013)
8. Ueno and Y. Minagawa et al., J. Biol. Chem. 289, 31212-31223 (2014)
9. Iida et al., J. Biol. Chem. In revision (2019)
10. Ando et al., ACS Photonics Under review (2019)

Biography

Ryota Iino received his Ph. D. degree in biophysics at Nagoya University, Japan in 2003. He worked as a research fellow at Japan Science and Technology Agency (2000-2005), a specially-appointed assistant professor (2005-2006) and an assistant professor at Osaka University (2006-2011), a lecturer (2011-2013) and an associate professor (2013-2014) at The University of Tokyo, Japan. In 2014, he began his independent academic career as a professor at Institute for Molecular Science (IMS), National Institutes of Natural Sciences (NINS), Japan. From 2018, he has also served as the director of Department of Life and Coordination-Complex Molecular Science, IMS, NINS.