Dear all,
2/4(二) 10:00 AM - 12:00 PM 將於中研院原分所浦大邦紀念講堂，舉辦Special Lectures on Advanced Bioimaging兩場學術演講。
因應2019新型冠狀病毒疫情，原分所將於演講前一天針對講堂公共區域進行消毒，演講當天於入口處提供酒精性乾洗手和設立健康監測站，並視當日出席人數採取間隔入座，保持講堂通風良好，希望大家可以安心參加這兩場學術演講。
演講當日，我們要求您務必戴上口罩，造成大家的不便，敬請見諒。
Please note that wearing a mask is mandatory. We are sorry for the inconvenience.
Special Lectures on Advanced Bioimaging
Date: February 4 (Tues.), 2020
Location: Dr. Poe Lecture Hall, IAMS, Academia Sinica
中央研究院原子與分子科學研究所 浦大邦紀念講堂 (臺大校園內)
10:00-11:00
Title: Optical Studies of Single Molecules and Single Gold Nanoparticles
Speaker: Michel Orrit (Institute of Physics, Leiden University, Netherlands)
Abstract:
Several optical methods give access to single molecules and single gold nanoparticles, whose strong interaction of light is mediated by the plasmon resonance: fluorescence (photoluminescence), scattering, absorption from photothermal contrast, refractive effects leading to shifts of the plasmon resonance, or plasmon-enhanced fluorescence of weak emitters.The plasmon-enhanced anti-Stokes photoluminescence of single gold nanorods provides their absolute temperature. Besides fluorescence, other optical signals can detect single molecules. The sensitivity of photothermal contrast can be pushed to the detection of photosensitive individual molecules such as organic conjugated polymers. Plasmonic gold nanorods are sensitive to refractive index changes in the environment. Non-absorbing protein molecules can thus be detected individually by their polarizability only, without need for fluorescent or absorbing labels. The binding and unbinding of single protein molecules from the solution give rise to sudden absorption steps, opening micro-analytical applications and in-situ sensing. Similar experiments can be done on single diffusing nanoparticles, without binding. Finally, plasmonic field enhancement in the near field of gold nanoparticles makes it possible to detect the fluorescence signals of weakly emitting absorbers such as dyes with low quantum yields for fluorescence. The redox cycles of single dye molecules or proteins can be monitored in real time.
11:00-12:00
Title: Advanced Concepts of Super-Resolution Fluorescence Microscopy
Speaker: Jörg Enderlein (Third Institute of Physics – Biophysics, Georg August University, Göttingen, Germany)
Abstract:
With the advent of super-resolution microscopy, the last ~25 years have seen a revolution in optical microscopy, pushing the spatial resolution capabilities of optical microscopy towards length scales that were typically accessible only by electron microscopy. In my presentation, I will give a short overview of the different principal approaches to super-resolution microscopy. I will briefly discuss the concepts of Structured Illumination Microscopy (SIM), Stimulated Emission Depletion (STED) microscopy, and Single Molecule Localization Microscopy (SMLM). Then, I will focus on two specific techniques where our group has contributed most. The first is Image Scanning Microscopy or ISM. This technique uses a simple combination of confocal microscopy with wide-field image detection for doubling the resolution of conventional microscopy. I will present the physical principals behind ISM, and the various kinds of its implementation. Meanwhile, ISM has found broad and wide applications and lies behind state-of-the-art commercial systems such as the extremely successful AiryScan microscope from Carl Zeiss Jena. The second is Metal-Induced Energy Transfer imaging or MIET imaging. I addresses the axial resolution in microscopy, which is particularly important for resolving three-dimensional structures. MIET is based on the intricate electrodynamic interaction of fluorescent emitters with metallic nanostructures. I will present the basic principles and several applications of this technique.